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15  janvier     09h09
Answer: centromere of cattle
   I would like to see better pure bioinformatics centromere predictors too. I only know of Centrominer which does not work well for me on various plant genomes. https: github.com aaranyue quarTeT Other than that I try to do repeat finding using EDTA, TRF, Red etc and piece it all together manually....
    04h59
Comment: BIOSZEN: R Shiny app for data analysis and visualization from Excel files
   Thank you very much for the feedback. I’ve now added a new gallery directory to the BIOSZEN GitHub repository with app screenshots and example outputs for each plot type (boxplot, barplot, violin, curves, stacked, correlation, etc.) so it’s easier to quickly see what the tool does before installing...
    00h24
Comment: BIOSZEN: R Shiny app for data analysis and visualization from Excel files
   Show some screenshots of your tool in the docs. Sell it to us. Convince us with some pretty plots that your tool is worth running. Few people have the time to go install a tool that they don’t know what it does.
    00h21
Comment: Blini: Lightweight nucleotide sequence search and dereplication
   One suggestion for your READMEe is to provide a realistic example. Say take the representative viral genome from NCBI, those reasonably small and demonstrate the tool via those examples. Right now your examples are input.fasta and output.csv and the reader has no idea what is inside these files,...
    00h14
Comment: Developing Shiny Apps with AI - online course in February
   Bumping threads solely for visibility is not acceptable on Biostar. This is against Biostar etiquette and makes it harder for others to get fair visibility. Posts should only be updated when there is new information or a substantive follow-up that adds important information not originally available...
14  janvier     22h59
BIOSZEN: R Shiny app for data analysis and visualization from Excel files
   I’m sharing BIOSZEN , a lightweight R Shiny application for data analysis and visualization based on Excel input files. It is designed for quick exploratory analysis and publication-ready plots without needing to write code. What BIOSZEN does Generates common plots from Excel tables: boxplots, bar...
    19h03
Blini: Lightweight nucleotide sequence search and dereplication
   Hi, I’m happy to share Blini , a new tool for sequence lookup and dereplication at massive scales, where traditional tools like BLAST or locally-run software might hit resource limits. What is Blini? Blini is an algorithm for quick nucleotide sequence lookup and dereplication. It combines several k...
    14h56
Comment: Which additional files are needed for the -taxids option in blast?
   You could do something like the following to extract fungal sequence entries from nr preformatted database (you will need to download the files for it) using a program provided in the blast package. blastdbcmd -db nr -taxids 4751 -outfmt %a -out fungal.fa then use makeblastdb to create your custom...
    13h41
Comment: Which additional files are needed for the -taxids option in blast?
   I am trying to do exactly this. I would like to make a custom database with proteins from Fungi, my taxids would be 4751. Would you mind sharing your code ?
    12h17
Comment: Are individual-mouse-based statistical tests possible with CellChat?
   You would need to run CellChat on every WT-KO individual mice pair to find that. I wonder whether it is meaningful to do that since usually you would want significant results that hold true in your group comparison, not in individual mice but not the group. What I would do (and have done before) is...
    12h10
Answer: Query regarding miRNA Expression Status
   From miRNAtissue atlas: https: web.ccb.uni-saarland.de mirnatissueatlas 2025 patterns hsa Atlas 2025 tissue rpmm mirna hsa-miR-383-5p yes organ no
    12h03
Are individual-mouse-based statistical tests possible with CellChat?
   Hey people, I asked this question on a few other forums too, but I think my chances of getting an answer on any of single forum are modest, so I want to ask here too, if that’s okay. Anyway, to the point: using CellChat (v2.2.0), when comparing between two groups (WT vs. KO,), each of which...
    11h46
De-noising OMIM phenotypes for multi-gene CNVs SVs: what heuristics do you use?
   I’m implementing CNV SV annotation and seeing a common issue: OMIM gene to phenotype mappings explode for multi-gene events and swamp the output. Right now we map overlapped genes to OMIM phenotypes and score them against case HPO terms, but we still get long, low-specificity lists. Inputs: CNV SV...
    09h02
Comment: Bioinformatician Atlanta USA
   Let me show you how to properly format a post.
    08h31
Query regarding miRNA Expression Status
   Hello I have a question regarding miRNA expression data. For example, I have information that hsa-miR-383-5p is downregulated in Ovarian Cancer cells. I want to know what is the status of this particular miRNA across all other tissues (whatever is available in the database). Eagerly waiting for...
    04h01
Quickly Build Your Own GEOmetadb with geokit
   If you are conducting bioinformatics research, especially when dealing with GEO (Gene Expression Omnibus) data, you may have used the [GEOmetadb](https: github.com zhujack GEOmetadb) package. While it was once a great tool, unfortunately, it has not been updated for years. Additionally, the...
13  janvier     23h32
Answer: Best tool to annotate open reading frames (ORFs) in virus metagenomics?
   I have never worked with Geneious Prime so what I am about to say might be my bias talking rather than an informed opinion. Commercial programs of that type are rarely best tools for anything other than being most convenient for the users who want to use a point-and-click interface. So count...
    21h24
Comment: Bioinformatician Atlanta USA
   www.p3scorp.com
    21h04
Best tool to annotate open reading frames (ORFs) in virus metagenomics?
   Hi all In your opinion, which is the best tool to anotate ORFs in viral metagenomics? I have some tick samples I want to do the viral characterization of, however I was told to annotate the ORFs with Geneious Prime, but I can t afford it so, which is the next best option?
    20h50
Answer: Writing all sequences to ’. BSgenome.Panubis.NCBI.Panubis1.0 inst extdata single
   I have solved it It was a corrupt installation of BSgenome. Here is the GitHub that helped me: https: github.com Bioconductor BSgenomeForge pull 46 [enter link description here][1] [1]: https: github.com Bioconductor BSgenomeForge pull 46
    20h23
Writing all sequences to ’. BSgenome.Panubis.NCBI.Panubis1.0 inst extdata single sequences.2bit’ ... Error in .TwoBits export(mapply(.DNAString to twoBit, object, seqnames), ...
   Hey everyone I followed this tutorial ([https: support.bioconductor.org p 125514 ][1]) in order to create a package for Papio anubis genome from NCBI. I originally wanted to use Ensembl’s assembly but BSgenome mainly takes UCSC and NCBI. Here is my seed file: Package: BSgenome.Panubis.NCBI.Panubis1...
    18h29
Answer: wf-trio (EPI2ME Desktop) - aggregate pileup gvcf failing with exit status 137 de
   W::bgzf read block] EOF marker is absent. The input may be truncated check the .g.vcf.gz files under home user epi2melabs instances wf-trio 01KEQ7QEFVGWP2B7SDZY9QCBQR work b8 66ca4c29765eee361abf0c4e9fa3f5 are not truncated. something like: find home user epi2melabs instances wf-trio...
    16h31
Answer: Ensembl genome browser is not working
   Hi all, My sincere apologies for the issues you are experiencing with Ensembl. We are aware of the site’s performance problems, which unfortunately have become more common as the codebase ages, datasets grow and our resources shift increasingly towards developing the new Ensembl site beta.ensembl...
    16h05
Comment: Bioinformatician Atlanta USA
   https: p3scorp.com jobs bioinformatician-scientist This link (or a similar one) may have been included in the original posting. It does not seem to be there now.
    16h03
Comment: Ensembl genome browser is not working
   Strange. This site considers it down for everyone. https: www.isitdownrightnow.com ensembl.org.html But it does work using Chrome for me too.